Composite
Part:BBa_K3710005:Design
Designed by: Sandra Lizeth Martinez Diaz, Erik Hanko and Katherine Baker Group: iGEM21_Manchester (2021-10-01)
Sarcosine Biosensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 87
Illegal AgeI site found at 773
Illegal AgeI site found at 885
Illegal AgeI site found at 2785 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal SapI site found at 190
Design Notes
SouR responds to sarcosine structurally related compounds (e.g. ethylglycine ). It is likely that creatinine or creatine could also act as an inducing ligand of SoxR.
We have included a Red Fluorescent Protein reporter to visualize when the sensor is active.
Source
The sequence of pSouR belongs to the intergenic region between the PA5415 (glyA1) and PA5414 genes of the sox operon of Pseudomonas aeruginosa that controls sarcosine metabolism.